The ribosomal stalk of the 60S subunit has been shown to play a crucial role in all steps of protein synthesis, but its structure and exact molecular function remain an unanswered question. In the present study, we show the low-resolution models of the solution structure of the yeast ribosomal stalk, composed of five proteins, P0–(P1–P2)2. The model of the pentameric stalk complex determined by small-angle X-ray scattering reveals an elongated shape with a maximum length of 13 nm. The model displays three distinct lobes, which may correspond to the individual P1–P2 heterodimers anchored to the C-terminal domain of the P0 protein.
Solution structure of the natively assembled yeast ribosomal stalk determined by small-angle X-ray scattering
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Przemysław Grela, Michal J. Gajda, Jean-Paul Armache, Roland Beckmann, Dawid Krokowski, Dmitri I. Svergun, Nikodem Grankowski, Marek Tchórzewski; Solution structure of the natively assembled yeast ribosomal stalk determined by small-angle X-ray scattering. Biochem J 1 June 2012; 444 (2): 205–209. doi: https://doi.org/10.1042/BJ20120115
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