Selenoproteins are proteins carrying the rare amino acid Sec (selenocysteine). Full expression of selenoproteins requires modification of tRNA[Ser]Sec, including N6-isopentenylation of base A37. We show that Trit1 is a dimethylallyl:tRNA[Ser]Sec transferase. Knockdown of Trit1 reduces expression of selenoproteins. Incubation of in vitro transcribed tRNA[Ser]Sec with recombinant Trit1 transfers [14C]dimethylallyl pyrophosphate to tRNA[Ser]Sec. 37A>G tRNA[Ser]Sec is resistant to isopentenylation by Trit1.
Mammalian Trit1 is a tRNA[Ser]Sec-isopentenyl transferase required for full selenoprotein expression
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Noelia Fradejas, Bradley A. Carlson, Eddy Rijntjes, Niels-Peter Becker, Ryuta Tobe, Ulrich Schweizer; Mammalian Trit1 is a tRNA[Ser]Sec-isopentenyl transferase required for full selenoprotein expression. Biochem J 1 March 2013; 450 (2): 427–432. doi: https://doi.org/10.1042/BJ20121713
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