We have shown previously that the polyamine spermidine is indispensable for differentiation of 3T3-L1 preadipocytes. In the present study, we examined the mechanism of spermidine function by using the polyamine biosynthesis inhibitor α-difluoromethylornithine in combination with the metabolically stable polyamine analogues γ-methylspermidine or (R,R)-α,ω-bismethylspermine. At the early phase of differentiation, spermidine-depleted 3T3-L1 cells showed decreased translation of the transcription factor C/EBPβ (CCAAT/enhancer-binding protein β), decreased PP2A (protein phosphatase 2A) activity and increased cytoplasmic localization of the RNA-binding protein HuR (human antigen R). The amount of HuR bound to C/EBPβ mRNA was reduced, whereas the amount of bound CUGBP2, an inhibitor of C/EBPβ translation, was increased. ANP32 (acidic nuclear phosphoprotein 32) proteins, which are known PP2A inhibitors and HuR ligands, bound more PP2A and HuR in spermidine-depleted than in control cells, whereas immunodepletion of ANP32 proteins from the lysate of spermidine-depleted cells restored PP2A activity. Taken together, our data shows that spermidine promotes C/EBPβ translation in differentiating 3T3-L1 cells, and that this process is controlled by the interaction of ANP32 with HuR and PP2A.
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Research Article|
July 12 2013
Spermidine promotes adipogenesis of 3T3-L1 cells by preventing interaction of ANP32 with HuR and PP2A
Mervi T. Hyvönen;
Mervi T. Hyvönen
1
*A.I. Virtanen Institute, University of Eastern Finland, Yliopistonranta 1E, 70210 Kuopio, Finland
1To whom correspondence should be addressed (email mervi.hyvonen@uef.fi).
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Taina Koponen;
Taina Koponen
*A.I. Virtanen Institute, University of Eastern Finland, Yliopistonranta 1E, 70210 Kuopio, Finland
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Janne Weisell;
Janne Weisell
†School of Pharmacy, Biocenter Kuopio, University of Eastern Finland, Yliopistonranta 1E, 70210 Kuopio, Finland
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Marko Pietilä;
Marko Pietilä
*A.I. Virtanen Institute, University of Eastern Finland, Yliopistonranta 1E, 70210 Kuopio, Finland
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Alex R. Khomutov;
Alex R. Khomutov
‡Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, 119991 Moscow, Russia
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Jouko Vepsäläinen;
Jouko Vepsäläinen
†School of Pharmacy, Biocenter Kuopio, University of Eastern Finland, Yliopistonranta 1E, 70210 Kuopio, Finland
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Leena Alhonen;
Leena Alhonen
*A.I. Virtanen Institute, University of Eastern Finland, Yliopistonranta 1E, 70210 Kuopio, Finland
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Tuomo A. Keinänen
Tuomo A. Keinänen
†School of Pharmacy, Biocenter Kuopio, University of Eastern Finland, Yliopistonranta 1E, 70210 Kuopio, Finland
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Biochem J (2013) 453 (3): 467–474.
Article history
Received:
February 20 2013
Revision Received:
April 22 2013
Accepted:
May 14 2013
Accepted Manuscript online:
May 14 2013
Citation
Mervi T. Hyvönen, Taina Koponen, Janne Weisell, Marko Pietilä, Alex R. Khomutov, Jouko Vepsäläinen, Leena Alhonen, Tuomo A. Keinänen; Spermidine promotes adipogenesis of 3T3-L1 cells by preventing interaction of ANP32 with HuR and PP2A. Biochem J 1 August 2013; 453 (3): 467–474. doi: https://doi.org/10.1042/BJ20130263
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