In Saccharomyces cerevisiae, synthesis of T6P (trehalose 6-phosphate) is essential for growth on most fermentable carbon sources. In the present study, the metabolic response to glucose was analysed in mutants with different capacities to accumulate T6P. A mutant carrying a deletion in the T6P synthase encoding gene, TPS1, which had no measurable T6P, exhibited impaired ethanol production, showed diminished plasma membrane H+-ATPase activation, and became rapidly depleted of nearly all adenine nucleotides which were irreversibly converted into inosine. Deletion of the AMP deaminase encoding gene, AMD1, in the tps1 strain prevented inosine formation, but did not rescue energy balance or growth on glucose. Neither the 90%-reduced T6P content observed in a tps1 mutant expressing the Tps1 protein from Yarrowia lipolytica, nor the hyperaccumulation of T6P in the tps2 mutant had significant effects on fermentation rates, growth on fermentable carbon sources or plasma membrane H+-ATPase activation. However, intracellular metabolite dynamics and pH homoeostasis were strongly affected by changes in T6P concentrations. Hyperaccumulation of T6P in the tps2 mutant caused an increase in cytosolic pH and strongly reduced growth rates on non-fermentable carbon sources, emphasizing the crucial role of the trehalose pathway in the regulation of respiratory and fermentative metabolism.
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Research Article|
August 09 2013
Metabolic phenotypes of Saccharomyces cerevisiae mutants with altered trehalose 6-phosphate dynamics Available to Purchase
Thomas Walther;
Thomas Walther
1
*Université de Toulouse, INSA, UPS, INP, 135 Avenue de Rangueil, F-31077 Toulouse, France
†INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France
‡CNRS, UMR5504, F-31400 Toulouse, France
1To whom correspondence should be addressed (email [email protected]).
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Narjes Mtimet;
Narjes Mtimet
*Université de Toulouse, INSA, UPS, INP, 135 Avenue de Rangueil, F-31077 Toulouse, France
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Ceren Alkim;
Ceren Alkim
*Université de Toulouse, INSA, UPS, INP, 135 Avenue de Rangueil, F-31077 Toulouse, France
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Amélie Vax;
Amélie Vax
*Université de Toulouse, INSA, UPS, INP, 135 Avenue de Rangueil, F-31077 Toulouse, France
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Marie-Odile Loret;
Marie-Odile Loret
*Université de Toulouse, INSA, UPS, INP, 135 Avenue de Rangueil, F-31077 Toulouse, France
†INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France
‡CNRS, UMR5504, F-31400 Toulouse, France
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Azmat Ullah;
Azmat Ullah
2
§Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, Faculty of Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands
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Carlos Gancedo;
Carlos Gancedo
∥Instituto de Investigaciones Biomedicas “Alberto Sols”, CSIC-UAM, Madrid 28029, Spain
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Gertien J. Smits;
Gertien J. Smits
§Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, Faculty of Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands
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Jean Marie François
Jean Marie François
*Université de Toulouse, INSA, UPS, INP, 135 Avenue de Rangueil, F-31077 Toulouse, France
†INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France
‡CNRS, UMR5504, F-31400 Toulouse, France
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Publisher: Portland Press Ltd
Received:
April 29 2013
Revision Received:
June 05 2013
Accepted:
June 14 2013
Accepted Manuscript online:
June 14 2013
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2013 Biochemical Society
2013
Biochem J (2013) 454 (2): 227–237.
Article history
Received:
April 29 2013
Revision Received:
June 05 2013
Accepted:
June 14 2013
Accepted Manuscript online:
June 14 2013
Citation
Thomas Walther, Narjes Mtimet, Ceren Alkim, Amélie Vax, Marie-Odile Loret, Azmat Ullah, Carlos Gancedo, Gertien J. Smits, Jean Marie François; Metabolic phenotypes of Saccharomyces cerevisiae mutants with altered trehalose 6-phosphate dynamics. Biochem J 1 September 2013; 454 (2): 227–237. doi: https://doi.org/10.1042/BJ20130587
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