The TenA protein family occurs in prokaryotes, plants and fungi; it has two subfamilies, one (TenA_C) having an active-site cysteine, the other (TenA_E) not. TenA_C proteins participate in thiamin salvage by hydrolysing the thiamin breakdown product amino-HMP (4-amino-5-aminomethyl-2-methylpyrimidine) to HMP (4-amino-5-hydroxymethyl-2-methylpyrimidine); the function of TenA_E proteins is unknown. Comparative analysis of prokaryote and plant genomes predicted that (i) TenA_E has a salvage role similar to, but not identical with, that of TenA_C and (ii) that TenA_E and TenA_C also have non-salvage roles since they occur in organisms that cannot make thiamin. Recombinant Arabidopsis and maize TenA_E proteins (At3g16990, GRMZM2G080501) hydrolysed amino-HMP to HMP and, far more actively, hydrolysed the N-formyl derivative of amino-HMP to amino-HMP. Ablating the At3g16990 gene in a line with a null mutation in the HMP biosynthesis gene ThiC prevented its rescue by amino-HMP. Ablating At3g16990 in the wild-type increased sensitivity to paraquat-induced oxidative stress; HMP overcame this increased sensitivity. Furthermore, the expression of TenA_E and ThiC genes in Arabidopsis and maize was inversely correlated. These results indicate that TenA_E proteins mediate amidohydrolase and aminohydrolase steps in the salvage of thiamin breakdown products. As such products can be toxic, TenA_E proteins may also pre-empt toxicity.
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October 2014
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Research Article|
September 08 2014
Salvage of the thiamin pyrimidine moiety by plant TenA proteins lacking an active-site cysteine
Rémi Zallot;
Rémi Zallot
*Microbiology and Cell Science Department, University of Florida, Gainesville, FL 32611, U.S.A.
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Mohammad Yazdani;
Mohammad Yazdani
†Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, U.S.A.
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Aymeric Goyer;
Aymeric Goyer
‡Department of Botany and Plant Pathology, Oregon State University, Hermiston, OR 97838, U.S.A.
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Michael J. Ziemak;
Michael J. Ziemak
§Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, U.S.A.
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Jiahn-Chou Guan;
Jiahn-Chou Guan
§Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, U.S.A.
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Donald R. McCarty;
Donald R. McCarty
§Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, U.S.A.
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Valérie de Crécy-Lagard;
Valérie de Crécy-Lagard
*Microbiology and Cell Science Department, University of Florida, Gainesville, FL 32611, U.S.A.
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Svetlana Gerdes;
Svetlana Gerdes
∥Mathematics and Computer Science Division, Argonne National Laboratory, Argonne, IL 60439, U.S.A.
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Timothy J. Garrett;
Timothy J. Garrett
¶College of Medicine, University of Florida, Gainesville, FL 32610, U.S.A.
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Jordi Benach;
Jordi Benach
1
**Department of Biological Sciences and Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, U.S.A.
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John F. Hunt;
John F. Hunt
**Department of Biological Sciences and Northeast Structural Genomics Consortium, Columbia University, New York, NY 10027, U.S.A.
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David K. Shintani;
David K. Shintani
†Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557, U.S.A.
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Andrew D. Hanson
Andrew D. Hanson
2
§Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, U.S.A.
2To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
April 25 2014
Revision Received:
June 30 2014
Accepted:
July 11 2014
Accepted Manuscript online:
July 11 2014
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2014 Biochemical Society
2014
Biochem J (2014) 463 (1): 145–155.
Article history
Received:
April 25 2014
Revision Received:
June 30 2014
Accepted:
July 11 2014
Accepted Manuscript online:
July 11 2014
Citation
Rémi Zallot, Mohammad Yazdani, Aymeric Goyer, Michael J. Ziemak, Jiahn-Chou Guan, Donald R. McCarty, Valérie de Crécy-Lagard, Svetlana Gerdes, Timothy J. Garrett, Jordi Benach, John F. Hunt, David K. Shintani, Andrew D. Hanson; Salvage of the thiamin pyrimidine moiety by plant TenA proteins lacking an active-site cysteine. Biochem J 1 October 2014; 463 (1): 145–155. doi: https://doi.org/10.1042/BJ20140522
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