Cell proliferation was inhibited following forced over-expression of miR-30a in the ovary cancer cell line A2780DX5 and the gastric cancer cell line SGC7901R. Interestingly, miR-30a targets the DNA replication protein RPA1, hinders the replication of DNA and induces DNA fragmentation. Furthermore, ataxia telangiectasia mutated (ATM) and checkpoint kinase 2 (CHK2) were phosphorylated after DNA damage, which induced p53 expression, thus triggering the S-phase checkpoint, arresting cell cycle progression and ultimately initiating cancer cell apoptosis. Therefore, forced miR-30a over-expression in cancer cells can be a potential way to inhibit tumour development.
miR-30a can inhibit DNA replication by targeting RPA1 thus slowing cancer cell proliferation
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Zhenyou Zou, Mengjie Ni, Jing Zhang, Yongfeng Chen, Hongyu Ma, Shihan Qian, Longhua Tang, Jiamei Tang, Hailun Yao, Chengbin Zhao, Xiongwen Lu, Hongyang Sun, Jue Qian, Xiaoting Mao, Xulin Lu, Qun Liu, Juping Zen, Hanbing Wu, Zhaosheng Bao, Shudan Lin, Hongyu Sheng, Yunlong Li, Yong Liang, Zhiqiang Chen, Dan Zong; miR-30a can inhibit DNA replication by targeting RPA1 thus slowing cancer cell proliferation. Biochem J 15 July 2016; 473 (14): 2131–2139. doi: https://doi.org/10.1042/BCJ20160177
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