Tetraspanins play important roles in normal (e.g. cell adhesion, motility, activation, and proliferation) and pathological conditions (e.g. metastasis and viral infection). Tetraspanins interact with integrins and regulate integrin functions, but the specifics of tetraspanin–integrin interactions are unclear. Using co-immunoprecipitation with integrins as a sole method to detect interaction between integrins and full-length tetraspanins, it has been proposed that the variable region (helices D and E) of the extracellular-2 (EC2) domain of tetraspanins laterally associates with a non-ligand-binding site of integrins. We describe that, using adhesion assays, the EC2 domain of CD81, CD9, and CD151 bound to integrin αvβ3, and this binding was suppressed by cRGDfV, a specific inhibitor of αvβ3, and antibody 7E3, which is mapped to the ligand-binding site of β3. We also present evidence that the specificity loop of β3 directly bound to the EC2 domains. This suggests that the EC2 domains specifically bind to the classical ligand-binding site of αvβ3. αvβ3 was a more effective receptor for the EC2 domains than the previously known tetraspanin receptors α3β1, α4β1, and α6β1. Docking simulation predicted that the helices A and B of CD81 EC2 bind to the RGD-binding site of αvβ3. Substituting Lys residues at positions 116 and 144/148 of CD81 EC2 in the predicted integrin-binding interface reduced the binding of CD81 EC2 to αvβ3, consistent with the docking model. These findings suggest that, in contrast with previous models, the ligand-binding site of integrin αvβ3, a new tetraspanin receptor, binds to the constant region (helices A and B) of the EC2 domain.
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February 2017
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Cover Image
Cover Image
Docking simulation predicts that the binding of CD81 extracellular domain-2 (EC2) to the RGD-binding site of integrin alphaVbeta3. CD81 EC2 (in red), integrin alphaV (in light green), and integrin beta3 (in purple). Please see pp. 589–596 for more information. Image provided by Yoshikazu Takada.
Research Article|
February 03 2017
The CD9, CD81, and CD151 EC2 domains bind to the classical RGD-binding site of integrin αvβ3 Available to Purchase
Jessica Yu;
Jessica Yu
1Department of Dermatology, Biochemistry and Molecular Medicine, University of California Davis School of Medicine, Sacramento, CA 95817, U.S.A.
2Ph.D. Program for Translational Medicine, College of Medical Sciences and Technology, Taipei Medical University, Taipei, Taiwan
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Chia-Ying Lee;
Chia-Ying Lee
2Ph.D. Program for Translational Medicine, College of Medical Sciences and Technology, Taipei Medical University, Taipei, Taiwan
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Chun Austin Changou;
Chun Austin Changou
2Ph.D. Program for Translational Medicine, College of Medical Sciences and Technology, Taipei Medical University, Taipei, Taiwan
3Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan
4Core Facility, Taipei Medical University, Taipei, Taiwan
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Dora M. Cedano-Prieto;
Dora M. Cedano-Prieto
1Department of Dermatology, Biochemistry and Molecular Medicine, University of California Davis School of Medicine, Sacramento, CA 95817, U.S.A.
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Yoko K. Takada;
Yoko K. Takada
1Department of Dermatology, Biochemistry and Molecular Medicine, University of California Davis School of Medicine, Sacramento, CA 95817, U.S.A.
2Ph.D. Program for Translational Medicine, College of Medical Sciences and Technology, Taipei Medical University, Taipei, Taiwan
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Yoshikazu Takada
1Department of Dermatology, Biochemistry and Molecular Medicine, University of California Davis School of Medicine, Sacramento, CA 95817, U.S.A.
2Ph.D. Program for Translational Medicine, College of Medical Sciences and Technology, Taipei Medical University, Taipei, Taiwan
Correspondence: Yoshikazu Takada ([email protected]; [email protected])
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Publisher: Portland Press Ltd
Received:
November 09 2016
Revision Received:
December 02 2016
Accepted:
December 19 2016
Accepted Manuscript online:
December 19 2016
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society
2017
Biochem J (2017) 474 (4): 589–596.
Article history
Received:
November 09 2016
Revision Received:
December 02 2016
Accepted:
December 19 2016
Accepted Manuscript online:
December 19 2016
Citation
Jessica Yu, Chia-Ying Lee, Chun Austin Changou, Dora M. Cedano-Prieto, Yoko K. Takada, Yoshikazu Takada; The CD9, CD81, and CD151 EC2 domains bind to the classical RGD-binding site of integrin αvβ3. Biochem J 15 February 2017; 474 (4): 589–596. doi: https://doi.org/10.1042/BCJ20160998
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