Flooding is an abiotic stress that creates hypoxic conditions triggered by redox potential leading to restricted growth and grain yield in plants. In the current study, we have investigated the effect of exogenous gibberellins (GA4+7) on soybean under flooding stress. A regulatory role of GAs on biochemical changes in soybean plants [including chlorophyll contents, endogenous bioactive GA1 and GA4, endogenous jasmonic acid (JA) and abscisic acid (ABA)] has been elucidated after 3 and 6 h of flooding stress. The modulation of stress-related bio-chemicals and their genetic determinants [for instance, ABA (Timing of CAB expression1—TOC1, ABA-receptor—ABAR) and NO (S-nitrosoglutathione reductase—GSNOR1, NO overproducer1—NOX, and nitrite reductase—NR)] in response to short-term flooding stress were also explored. The current study showed that exogenous GAs rescued chlorophyll contents, enhanced endogenous bioactive GA1 and GA4 levels, endogenous jasmonic acid (JA) and checked the rate of ABA biosynthesis under short-term flooding. The exo-GAs induced the glutathione activity and reduced the resulting superoxide anion contents during short-term flooding in Pungsannamul soybean. Exo-GAs also triggered the endogenous S-nitrosothiols (precursor for increased NO production) that have been decreased over the time. Moreover, the exo-GAs could impinge a variety of biochemical and transcriptional programs that are ameliorative to plant growth during short-term flooding stress. The presence of GA1 and GA4 also confirms the presence of both C13-hydroxylation pathway and non-C13-hydroxylation pathway in soybean, respectively.
In this issue of the Biochemical Journal, Thomas et al. report on the interaction between AMPK and one of its target proteins TBC1D1. The research shows that the association is AMPK-isoform-specific and that it is disrupted by a mutation linked to obesity. The cover image, taken from the article, shows Western blot analysis of the phosphorylation of transiently expressed GFP-TBC1D1 as well as AMPK and ACC from Flp-In HEK293 cells that stably express FLAG-AMPK-α1 or FLAG-AMPK-α2. For further details, see pages 2969–2983.
Gibberellin application ameliorates the adverse impact of short-term flooding on Glycine max L.
Muhammad Aaqil Khan, Muhammad Hamayun, Amjad Iqbal, Sumera Afzal Khan, Anwar Hussain, Sajjad Asaf, Abdul Latif Khan, Byung-Wook Yun, In-Jung Lee; Gibberellin application ameliorates the adverse impact of short-term flooding on Glycine max L.. Biochem J 28 September 2018; 475 (18): 2893–2905. doi: https://doi.org/10.1042/BCJ20180534
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