The MarR family is unique to both bacteria and archaea. The members of this family, one of the most prevalent families of transcriptional regulators in bacteria, enable bacteria to adapt to changing environmental conditions, such as the presence of antibiotics, toxic chemicals, or reactive oxygen species (ROS), mainly by thiol-disulfide switches. Although the genome of Corynebacterium glutamicum encodes a large number of the putative MarR-type transcriptional regulators, their physiological and biochemical functions have so far been limited to only two proteins, regulator of oxidative stress response RosR and quinone oxidoreductase regulator QosR. Here, we report that the ncgl2617 gene (cosR) of C. glutamicum encoding an MarR-type transcriptional regulator plays an important role in oxidative stress resistance. The cosR null mutant is found to be more resistant to various oxidants and antibiotics, accompanied by a decrease in ROS production and protein carbonylation levels under various stresses. Protein biochemical function analysis shows that two Cys residues presenting at 49 and 62 sites in CosR are redox-active. They form intermolecular disulfide bonds in CosR under oxidative stress. This CosR oxidation leads to its dissociation from promoter DNA, depression of the target DNA, and increased oxidative stress resistance of C. glutamicum. Together, the results reveal that CosR is a redox-sensitive regulator that senses peroxide stress to mediate oxidative stress resistance in C. glutamicum.
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December 2018
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In this issue, Arnal et al. investigate the molecular basis of substrate regiospecificity and processivity of an exemplar bacterial Glycoside Hydrolase Family 74 endo-xyloglucanase. The cover image shows a protein structure with a uniquely large oligosaccharide complex. The enzyme was taken from a wheat rhizome bacterium. The image was supplied by Harry Brumer and Peter Stogios.
Research Article|
December 19 2018
CosR is an oxidative stress sensing a MarR-type transcriptional repressor in Corynebacterium glutamicum
Meiru Si
;
Meiru Si
*
1College of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China
Correspondence: Meiru Si ([email protected]) or Can Chen ([email protected])
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Can Chen;
Can Chen
*
2College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, Henan 466001, China
Correspondence: Meiru Si ([email protected]) or Can Chen ([email protected])
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Tao Su;
Tao Su
*
1College of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China
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Chengchuan Che;
Chengchuan Che
1College of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China
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Shumin Yao;
Shumin Yao
1College of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China
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Guangjie Liang;
Guangjie Liang
1College of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China
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Guizhi Li;
Guizhi Li
1College of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China
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Ge Yang
Ge Yang
1College of Life Sciences, Qufu Normal University, Qufu, Shandong 273165, China
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Publisher: Portland Press Ltd
Received:
August 20 2018
Revision Received:
November 12 2018
Accepted:
November 23 2018
Accepted Manuscript online:
November 26 2018
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2018
Biochem J (2018) 475 (24): 3979–3995.
Article history
Received:
August 20 2018
Revision Received:
November 12 2018
Accepted:
November 23 2018
Accepted Manuscript online:
November 26 2018
Citation
Meiru Si, Can Chen, Tao Su, Chengchuan Che, Shumin Yao, Guangjie Liang, Guizhi Li, Ge Yang; CosR is an oxidative stress sensing a MarR-type transcriptional repressor in Corynebacterium glutamicum. Biochem J 21 December 2018; 475 (24): 3979–3995. doi: https://doi.org/10.1042/BCJ20180677
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