Regulation of nucleotide and nucleoside concentrations is critical for faithful DNA replication, transcription, and translation in all organisms, and has been linked to bacterial biofilm formation. Unusual 2′,3′-cyclic nucleotide monophosphates (2′,3′-cNMPs) recently were quantified in mammalian systems, and previous reports have linked these nucleotides to cellular stress and damage in eukaryotes, suggesting an intriguing connection with nucleotide/nucleoside pools and/or cyclic nucleotide signaling. This work reports the first quantification of 2′,3′-cNMPs in Escherichia coli and demonstrates that 2′,3′-cNMP levels in E. coli are generated specifically from RNase I-catalyzed RNA degradation, presumably as part of a previously unidentified nucleotide salvage pathway. Furthermore, RNase I and 2′,3′-cNMP levels are demonstrated to play an important role in controlling biofilm formation. This work identifies a physiological role for cytoplasmic RNase I and constitutes the first progress toward elucidating the biological functions of bacterial 2′,3′-cNMPs.
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April 2018
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Confocal laser scanning microscopy of HeLa cells transfected with FLAG-ITSN1-s, stained with anti-FLAG (green), anti-Lamin A/C (red) and DAPI (blue). In this issue of the Biochemical Journal, Alvisi et al. discuss the behaviour of intersectin as an endocytic protein; for details see pages 1455–1472.
Research Article|
April 30 2018
RNase I regulates Escherichia coli 2′,3′-cyclic nucleotide monophosphate levels and biofilm formation
Benjamin M. Fontaine;
Benjamin M. Fontaine
1Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, U.S.A.
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Kevin S. Martin;
Kevin S. Martin
1Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, U.S.A.
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Jennifer M. Garcia-Rodriguez;
Jennifer M. Garcia-Rodriguez
1Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, U.S.A.
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Claire Jung;
Claire Jung
1Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, U.S.A.
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Laura Briggs;
Laura Briggs
1Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, U.S.A.
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Jessica E. Southwell;
Jessica E. Southwell
1Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, U.S.A.
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Xin Jia;
Xin Jia
1Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, U.S.A.
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Emily E. Weinert
1Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, U.S.A.
Correspondence: Emily E. Weinert (emily.weinert@emory.edu)
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Biochem J (2018) 475 (8): 1491–1506.
Article history
Received:
November 29 2017
Revision Received:
March 13 2018
Accepted:
March 16 2018
Accepted Manuscript online:
March 19 2018
Citation
Benjamin M. Fontaine, Kevin S. Martin, Jennifer M. Garcia-Rodriguez, Claire Jung, Laura Briggs, Jessica E. Southwell, Xin Jia, Emily E. Weinert; RNase I regulates Escherichia coli 2′,3′-cyclic nucleotide monophosphate levels and biofilm formation. Biochem J 30 April 2018; 475 (8): 1491–1506. doi: https://doi.org/10.1042/BCJ20170906
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