Eukaryotic DNA replication is a highly dynamic and tightly regulated process. Replication involves several dozens of replication proteins, including the initiators ORC and Cdc6, replicative CMG helicase, DNA polymerase α-primase, leading-strand DNA polymerase ε, and lagging-strand DNA polymerase δ. These proteins work together in a spatially and temporally controlled manner to synthesize new DNA from the parental DNA templates. During DNA replication, epigenetic information imprinted on DNA and histone proteins is also copied to the daughter DNA to maintain the chromatin status. DNA methyltransferase 1 is primarily responsible for copying the parental DNA methylation pattern into the nascent DNA. Epigenetic information encoded in histones is transferred via a more complex and less well-understood process termed replication-couple nucleosome assembly. Here, we summarize the most recent structural and biochemical insights into DNA replication initiation, replication fork elongation, chromatin assembly and maintenance, and related regulatory mechanisms.
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September 2020
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Cover Image
Cover Image
Thermogenesis of Arum maculatum. The cover of this issue of the Biochemical Journal features an image from Ito et al., who report the temperature-inducible degradation of AOX proteins in mitochondria from appendices of A. maculatum. Image courtesy of Kikukatsu Ito.
Review Article|
September 24 2020
Molecular mechanisms of eukaryotic origin initiation, replication fork progression, and chromatin maintenance
Zuanning Yuan;
Structural Biology Program, Van Andel Institute, Grand Rapids, Michigan, U.S.A
Correspondence: Zuanning Yuan ([email protected]) or Huilin Li ([email protected])
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Huilin Li
Structural Biology Program, Van Andel Institute, Grand Rapids, Michigan, U.S.A
Correspondence: Zuanning Yuan ([email protected]) or Huilin Li ([email protected])
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
July 22 2020
Revision Received:
August 29 2020
Accepted:
September 04 2020
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2020
Biochem J (2020) 477 (18): 3499–3525.
Article history
Received:
July 22 2020
Revision Received:
August 29 2020
Accepted:
September 04 2020
Citation
Zuanning Yuan, Huilin Li; Molecular mechanisms of eukaryotic origin initiation, replication fork progression, and chromatin maintenance. Biochem J 30 September 2020; 477 (18): 3499–3525. doi: https://doi.org/10.1042/BCJ20200065
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