Candida albicans is an opportunist pathogen responsible for a large spectrum of infections, from superficial mycosis to systemic diseases known as candidiasis. During infection in vivo, Candida albicans must adapt to host microenvironments and this adaptive response is crucial for the survival of this organism, as it facilitates the effective assimilation of alternative carbon sources others than glucose. We performed a global proteomic analysis on the global changes in protein abundance in response to changes in micronutrient levels, and, in parallel, explored changes in the intracellular redox and metabolic status of the cells. We show here that each of the carbon sources considered — glucose, acetate and lactate — induces a unique pattern of response in C. albicans cells, and that some conditions trigger an original and specific adaptive response involving the adaptation of metabolic pathways, but also a complete remodeling of thiol-dependent antioxidant defenses. Protein S-thiolation and the overproduction of reduced glutathione are two components of the response to high glucose concentration. In the presence of acetate, glutathione-dependent oxidative stress occurs, reduced thiol groups bind to proteins, and glutathione is exported out of the cells, these changes probably being triggered by an increase in glutathione-S-transferases. Overall, our results suggest that the role of cellular redox status regulation and defenses against oxidative stress, including the thiol- and glutathione-dependent response, in the adaptive response of C. albicans to alternative carbon sources should be reconsidered.
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February 2023
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Research Article|
February 07 2023
The adaptive response to alternative carbon sources in the pathogen Candida albicans involves a remodeling of thiol- and glutathione-dependent redox status
Juliette Bayot;
Juliette Bayot
Data curation, Formal analysis
1Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France
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Caroline Martin;
Caroline Martin
Data curation, Methodology
1Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France
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Guillaume Chevreux;
Guillaume Chevreux
Data curation, Software, Formal analysis
1Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France
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Jean-Michel Camadro;
Jean-Michel Camadro
Data curation, Writing - review & editing
1Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France
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Françoise Auchère
1Université Paris Cité, CNRS, Institut Jacques Monod, F-75013 Paris, France
Correspondence: Françoise Auchère ([email protected])
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Publisher: Portland Press Ltd
Received:
October 10 2022
Revision Received:
January 03 2023
Accepted:
January 10 2023
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society
2023
Biochem J (2023) 480 (3): 197–217.
Article history
Received:
October 10 2022
Revision Received:
January 03 2023
Accepted:
January 10 2023
Citation
Juliette Bayot, Caroline Martin, Guillaume Chevreux, Jean-Michel Camadro, Françoise Auchère; The adaptive response to alternative carbon sources in the pathogen Candida albicans involves a remodeling of thiol- and glutathione-dependent redox status. Biochem J 15 February 2023; 480 (3): 197–217. doi: https://doi.org/10.1042/BCJ20220505
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