1. The cobalamin-independent synthesis of methionine from serine and homocysteine by ultrasonic extracts of E. coli with tetrahydropteroyltriglutamate as cofactor was inhibited competitively by tetrahydropteroylmonoglutamate and derivatives which were readily converted into this compound. 2. The potency of these inhibitors was directly related to their ability to function as cofactors or substrates in the alternative, cobalamin- dependent mechanism for homocysteine methylation. 3. The cobalamin-dependent and -independent mechanisms of homocysteine methylation were both inhibited by reduced derivatives of aminopterin in a similar manner. 4. It was tentatively concluded that the inhibition was due to a competitive interaction between the folates for N(5)N(10)-methylenetetrahydrofolate reductase.
Skip Nav Destination
Research Article| November 01 1965
The interaction of folic acid derivatives in the methylation of homocysteine
Biochem J (1965) 97 (2): 500–512.
- Views Icon Views
- Share Icon Share
JR Guest, DD Woods; The interaction of folic acid derivatives in the methylation of homocysteine. Biochem J 1 November 1965; 97 (2): 500–512. doi: https://doi.org/10.1042/bj0970500
Download citation file:
Don't already have an account? Register
Get Access To This Article
Buy This Article