Pseudomonas fluorescens N.C.I.B. 8248 was adapted to grow on potassium d-glucose 6-O-sulphate as the sole carbon and sulphur source. Adapted bacteria grew optimally at 37°C on 1.6% (w/v) sulphate ester and growth coincided with the disappearance of the ester from the culture medium at a rate of 2.4mg/h per ml. Three sulphated compounds were detected in the culture fluid at the termination of growth. One of these was present in traces only and has not been identified. The second was present in somewhat greater amounts and was identified as the 6-O-sulphate ester of d-gluconate, and the major metabolite was identified as d-glycerate 3-O-sulphate. Sulphur utilization by the organism was not associated with the appearance of a glycosulphatase enzyme in the cells. However, a novel enzyme system (or systems) was present that liberated inorganic 35SO42− ions from dipotassium d-gluconate 6[35S]-O-sulphate and from dipotassium dl-glycerate 3[35S]-O-sulphate. Activity towards the latter substrate could not be detected when the adapted or parent Pseudomonas strain was cultured on d-glucose and potassium sulphate as respective carbon and sulphur sources. Some properties of the enzyme acting on the glycerate ester are recorded.
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Research Article|
February 01 1971
Sulphur utilization during growth of Pseudomonas fluorescens on potassium d-glucose 6-O-sulphate
J. W. Fitzgerald;
J. W. Fitzgerald
1Department of Biochemistry, University College, P.O. Box 78, Cardiff CF1 1XL, U.K.
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K. S. Dodgson
K. S. Dodgson
1Department of Biochemistry, University College, P.O. Box 78, Cardiff CF1 1XL, U.K.
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Publisher: Portland Press Ltd
© 1971 The Biochemical Society
1971
Biochem J (1971) 121 (3): 521–528.
Citation
J. W. Fitzgerald, K. S. Dodgson; Sulphur utilization during growth of Pseudomonas fluorescens on potassium d-glucose 6-O-sulphate. Biochem J 1 February 1971; 121 (3): 521–528. doi: https://doi.org/10.1042/bj1210521
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