A general equation is derived describing data of DNA-RNA hybridization in the presence of a competing self-annealing reaction of RNA. The well known double-reciprocal relation and the Scatchard equation are shown to be limiting cases of this general equation. Some new hybridization data at various temperatures are presented and analysed by using the new equation. The results can only be explained if we assume that the behavior of DNA towards single RNA molecules is the same as that towards the annealed form, (RNA12. The variation of the equilibrium constant of the hybridization reaction with temperature is small, indicating a small heat of reaction. The maximum amount of hybridized RNA at equilibrium appears to be independent of temperature.
Skip Nav Destination
Article navigation
February 1976
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Research Article|
February 01 1976
The theory of the deoxyribonucleic acid - ribonucleic acid hybridization reaction
H Thomou;
H Thomou
1Department of Biology, N.R.C. ‘Democritos’, Aghia Paraskevi, Athens, Greece, and Physical Chemistry Laboratory, University of Patras, Patras, Greece
Search for other works by this author on:
N A Katsanos
N A Katsanos
1Department of Biology, N.R.C. ‘Democritos’, Aghia Paraskevi, Athens, Greece, and Physical Chemistry Laboratory, University of Patras, Patras, Greece
Search for other works by this author on:
Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© London: 1976 The Biochemical Society
1976
Biochem J (1976) 153 (2): 241–247.
Citation
H Thomou, N A Katsanos; The theory of the deoxyribonucleic acid - ribonucleic acid hybridization reaction. Biochem J 1 February 1976; 153 (2): 241–247. doi: https://doi.org/10.1042/bj1530241
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.