The process leading to the rise of acetyl-CoA carboxylase activity in rat mammary tissue after the onset of lactation was investigated. The kinetics of change in enzyme activity and enzyme immunotitratable with antibody against avian liver acetyl-CoA carboxylase were determined during the course of lactogenic differentiation. The antibody inactivates and specifically precipitates acetyl-CoA carboxylase from rat mammary tissue as well as that from chicken liver cytosol. Characterization of the immunoprecipitate of the mammary tissue carboxylase by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis reveals a single biotin-containing polypeptide of about 230000mol.wt. This molecular weight is approximately twice that reported for the avian liver enzyme. However, chicken liver cytosol prepared in the presence of trypsin inhibitor and subjected to immunoprecipitation gives rise to a biotin-containing subunit of 230000mol.wt. as determined by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis; omission of proteinase inhibitor leads to a subunit(s) approximately one-half this size. Throughout gestation both carboxylase activity and amounts of immunotitratable enzyme remained low; however, after parturition both parameters rose concomitantly to values 30–40 times the initial values. Therefore the elevated concentration of acetyl-CoA carboxylase appears to result from an increased rate of synthesis of enzyme relative to degradation rather than to activation of a pre-existing form of the enzyme.
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Research Article|
March 15 1977
Changes in mammary-gland acetyl-coenzyme A carboxylase associated with lactogenic differentiation
Julia C. Mackall;
Julia C. Mackall
1Department of Physiological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.
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M. Daniel Lane
M. Daniel Lane
1Department of Physiological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1977 London: The Biochemical Society
1977
Biochem J (1977) 162 (3): 635–642.
Citation
Julia C. Mackall, M. Daniel Lane; Changes in mammary-gland acetyl-coenzyme A carboxylase associated with lactogenic differentiation. Biochem J 15 March 1977; 162 (3): 635–642. doi: https://doi.org/10.1042/bj1620635
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