The transcription factors Oct-1 and Oct-2 bind differentially to three octamer binding sequences corresponding to the octamer binding site from the H2B promoter [ATGCTAATAA], a simple TAATGARAT motif, found in herpes simplex virus IE4/5 genes [GCGGTAATGAGAT], and a perfect consensus overlapping octamer/TAATGARAT motif [ATGCTAATGAGAT]. By comparing the effects of protein kinase A, protein kinase C and casein kinase 2 in vitro on the binding of Oct-1 and Oct-2 to the three motifs, we show that the actions of these kinases regulate Oct-1 and Oct-2 DNA binding independently of each other in a binding-site-specific manner. Inhibition of cellular phosphatases also regulate Oct-1 and Oct-2 DNA binding in a binding-site-specific manner. Both kinase and phosphatase activity are important for regulating the DNA binding activity of Oct-1 and Oct-2 because, in the presence of phosphatase inhibitors, protein kinase A attenuates the binding of both Oct-1 and Oct-2 to the octamer binding site but enhances binding when phosphatase inhibitors are omitted. Thus the DNA specificity of Oct-1 and Oct-2 can be regulated in vitro by the action of different kinases.
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Research Article|
May 01 1996
Oct-1 [corrected] and Oct-2 DNA-binding site specificity is regulated in vitro by different kinases
Stephanie J. GRENFELL;
Stephanie J. GRENFELL
*Department of Haematology, University College London Medical School, 98 Chenies Mews, London WC1E 6HX, U.K.
†Department of Molecular Pathology, University College London Medical School, The Windeyer Building, 46 Cleveland Street, London, W1P 6DB, U.K.
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David S. LATCHMAN;
David S. LATCHMAN
†Department of Molecular Pathology, University College London Medical School, The Windeyer Building, 46 Cleveland Street, London, W1P 6DB, U.K.
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N. Shaun B. THOMAS
N. Shaun B. THOMAS
‡
*Department of Haematology, University College London Medical School, 98 Chenies Mews, London WC1E 6HX, U.K.
‡To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
September 01 1995
Revision Received:
December 01 1995
Accepted:
December 22 1995
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 315 (3): 889–893.
Article history
Received:
September 01 1995
Revision Received:
December 01 1995
Accepted:
December 22 1995
Connected Content
A correction has been published:
CT-1 and Oct-2 DNA-binding site specificity is regulated in vitro by different kinases
Citation
Stephanie J. GRENFELL, David S. LATCHMAN, N. Shaun B. THOMAS; Oct-1 [corrected] and Oct-2 DNA-binding site specificity is regulated in vitro by different kinases. Biochem J 1 May 1996; 315 (3): 889–893. doi: https://doi.org/10.1042/bj3150889
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