Lipoprotein lipase (LPL) activity was studied in rat cardiomyocytes after overnight culture (16 h) in the presence of insulin (100 nM) and/or dexamethasone (100 nM). Insulin in combination with dexamethasone (INS/DEX) increased heparin-releasable LPL activity by 71% over the control level (566±85 versus 331±48 nmol/h·mg cell protein). This was accompanied by a 61% increase in total cellular LPL activity (914±89 versus 567±64 nmol/h·mg cell protein). The increase in LPL activity occurred at sub-nanomolar concentrations of the hormones, but neither hormone was effective alone. LPL protein mass, quantified by ELISA, was the same in both control and INS/DEX-treated cells (27.7 versus 28.6 ng/mg cell protein, respectively), thus LPL specific activity in cardiomyocytes was increased by INS/DEX treatment (0.113 versus 0.069 mU/ng LPL protein). These findings emphasize the importance of hormonal interactions in the regulation of LPL in heart tissue.
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October 1997
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Research Article|
October 15 1997
Lipoprotein lipase activity in rat cardiomyocytes is stimulated by insulin and dexamethasone
H. Stephen EWART;
H. Stephen EWART
1The University of Calgary, Department of Pharmacology and Therapeutics, 3330 Hospital Drive N. W., Calgary, AB, T2N 4N1, Canada
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Rogayah CARROLL;
Rogayah CARROLL
1The University of Calgary, Department of Pharmacology and Therapeutics, 3330 Hospital Drive N. W., Calgary, AB, T2N 4N1, Canada
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L. David SEVERSON
L. David SEVERSON
1
1The University of Calgary, Department of Pharmacology and Therapeutics, 3330 Hospital Drive N. W., Calgary, AB, T2N 4N1, Canada
1To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
March 06 1997
Revision Received:
May 15 1997
Accepted:
June 19 1997
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1997
1997
Biochem J (1997) 327 (2): 439–442.
Article history
Received:
March 06 1997
Revision Received:
May 15 1997
Accepted:
June 19 1997
Citation
H. Stephen EWART, Rogayah CARROLL, L. David SEVERSON; Lipoprotein lipase activity in rat cardiomyocytes is stimulated by insulin and dexamethasone. Biochem J 15 October 1997; 327 (2): 439–442. doi: https://doi.org/10.1042/bj3270439
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