Mouse ODC (ornithine decarboxylase) is quickly degraded by the 26S proteasome in mammalian and fungal cells. Its degradation is independent of ubiquitin but requires a degradation signal composed of residues 425–461 at the ODC C-terminus, cODC (the last 37 amino acids of the ODC C-terminus). Mutational analysis of cODC revealed the presence of two essential elements in the degradation signal. The first consists of cysteine and alanine at residues 441 and 442 respectively. The second element is the C-terminus distal to residue 442; it has little or no sequence specificity, but is intolerant of insertions or deletions that alter its span. Reducing conditions, which preclude all well-characterized chemical reactions of the Cys441 thiol, are essential for in vitro degradation. These experiments imply that the degradative function of Cys441 does not involve its participation in chemical reaction; it, instead, functions within a structural element for recognition by the 26S proteasome.
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March 2008
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Research Article|
February 12 2008
Structural elements of the ubiquitin-independent proteasome degron of ornithine decarboxylase
Junko Takeuchi;
Junko Takeuchi
1Department of Microbiology and Immunology, University of California, San Francisco, CA 94143, U.S.A.
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Hui Chen;
Hui Chen
1
1Department of Microbiology and Immunology, University of California, San Francisco, CA 94143, U.S.A.
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Martin A. Hoyt;
Martin A. Hoyt
1Department of Microbiology and Immunology, University of California, San Francisco, CA 94143, U.S.A.
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Philip Coffino
Philip Coffino
2
1Department of Microbiology and Immunology, University of California, San Francisco, CA 94143, U.S.A.
2To whom correspondence should be addressed (email philip.coffino@ucsf.edu).
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Publisher: Portland Press Ltd
Received:
September 11 2007
Revision Received:
October 30 2007
Accepted:
November 02 2007
Accepted Manuscript online:
November 02 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biochem J (2008) 410 (2): 401–407.
Article history
Received:
September 11 2007
Revision Received:
October 30 2007
Accepted:
November 02 2007
Accepted Manuscript online:
November 02 2007
Citation
Junko Takeuchi, Hui Chen, Martin A. Hoyt, Philip Coffino; Structural elements of the ubiquitin-independent proteasome degron of ornithine decarboxylase. Biochem J 1 March 2008; 410 (2): 401–407. doi: https://doi.org/10.1042/BJ20071239
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