We have previously reported that, in neuroblastoma LAN-5 cells, calpastatin is in an aggregated state, close to the cell nucleus [De Tullio, Passalacqua, Averna, Salamino, Melloni and Pontremoli (1999) Biochem. J. 343, 467–472]. In the present paper, we demonstrate that aggregated calpastatin is predominantly in a phosphorylated state. An increase in intracellular free [Ca2+] induces both dephosphorylation of calpastatin, through the action of a phosphoprotein phosphatase, and its redistribution as a soluble inhibitor species. cAMP, but not PMA-induced phosphorylation, reverses calpastatin distribution favouring its aggregation. This intracellular reversible mechanism, regulating the level of cytosolic calpastatin, could be considered a strategy through which calpain can escape calpastatin inhibition, especially during earlier steps of its activation process.
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February 2001
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Research Article|
February 08 2001
Changes in intracellular calpastatin localization are mediated by reversible phosphorylation
Monica AVERNA;
Monica AVERNA
1Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV 1, 16132 Genoa, Italy
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Roberta DE TULLIO;
Roberta DE TULLIO
1Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV 1, 16132 Genoa, Italy
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Mario PASSALACQUA;
Mario PASSALACQUA
1Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV 1, 16132 Genoa, Italy
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Franca SALAMINO;
Franca SALAMINO
1Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV 1, 16132 Genoa, Italy
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Sandro PONTREMOLI;
Sandro PONTREMOLI
1Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV 1, 16132 Genoa, Italy
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Edon MELLONI
Edon MELLONI
1
1Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV 1, 16132 Genoa, Italy
1To whom correspondence should be addressed (e-mail melloni@csita.unige.it).
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Publisher: Portland Press Ltd
Received:
July 26 2000
Revision Received:
October 30 2000
Accepted:
November 21 2000
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2001
2001
Biochem J (2001) 354 (1): 25–30.
Article history
Received:
July 26 2000
Revision Received:
October 30 2000
Accepted:
November 21 2000
Citation
Monica AVERNA, Roberta DE TULLIO, Mario PASSALACQUA, Franca SALAMINO, Sandro PONTREMOLI, Edon MELLONI; Changes in intracellular calpastatin localization are mediated by reversible phosphorylation. Biochem J 15 February 2001; 354 (1): 25–30. doi: https://doi.org/10.1042/bj3540025
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