Rats were injected with [3H]leucine, and at various times thereafter labelled albumin was isolated by electrophoresis from their livers and blood plasma. The specific radioactivity of each protein was determined by spectrophotometry and liquid-scintillation spectrometry. Intrahepatic albumin was shown to be identical with plasma albumin by its electrophoretic mobility and antigenicity. It was found that intrahepatic albumin was the direct precursor of plasma albumin. Comparison of their specific radioactivities showed that intrahepatic albumin attained a higher specific radioactivity before plasma albumin. When plasma albumin reached its maximum specific radioactivity, that of intrahepatic albumin had decreased to a similar value. Thereafter, the specific radioactivity of intrahepatic albumin remained lower than that of plasma albumin.
Research Article| January 01 1968
Precursor–product relationship between intrahepatic albumin and plasma albumin
Biochem J (1968) 106 (2): 503–506.
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A. V. LeBouton; Precursor–product relationship between intrahepatic albumin and plasma albumin. Biochem J 1 January 1968; 106 (2): 503–506. doi: https://doi.org/10.1042/bj1060503
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