An improved method for the isolation of normal immunoglobulin E is described. The method is based on the use of an immunoadsorbent formed by the mechanical entrapment of antibodies against a myeloma immunoglobulin E into a lattice of a highly cross-linked macroporous polyacrylamide gel. Normal immunoglobulin E was isolated from an immunoglobulin E-rich serum pool as well as from an individual healthy donor. The isolated immunoglobulin E was contaminated with about 20% of immunoglobulin G. An antiserum against normal immunoglobulin E was prepared by immunizing rabbits with the isolated immunoglobulin E.
Research Article| May 01 1971
Isolation of normal immunoglobulin E by means of an immunoadsorbent
Biochem J (1971) 122 (4): 405–408.
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S. Carrel, Lore Theilkäs, A. Morell, F. Skvaril, S. Barandun; Isolation of normal immunoglobulin E by means of an immunoadsorbent. Biochem J 1 May 1971; 122 (4): 405–408. doi: https://doi.org/10.1042/bj1220405
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