When cells of Pseudomonas are grown on citrate as the sole carbon source they oxidize citrate and isocitrate rapidly. Fluorocitrate inhibits the oxidation of citrate. Fluorocitrate-treated cells accumulate [6-14C]citrate, as shown by a rapid Millipore-filtration technique. In the absence of fluorocitrate most of the [6-14C]-citrate is lost in the form of14CO2. The isolation of a pseudomonad characterized by its ability to grow on tricarballylate as a sole carbon source has facilitated the study of the tricarboxylate-carrier specificity. Cells grown on citrate will exchange radioactive citrate for unlabelled citrate or isocitrate but not for cis-aconitate, trans-aconitate or tricarballylate. Cells grown on tricarballylate will exchange radioactive citrate for unlabelled citrate, cis-aconitate or tricarballylate, but not for isocitrate or trans-aconitate. The properties of the exchange system involved are compared with those of the related system in mitochondria.
Research Article|July 01 1971
The transport of citrate and other tricarboxylic acids in two species of Pseudomonas
H. G. Lawford;
Biochem J (1971) 123 (4): 571-577.
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H. G. Lawford, G. R. Williams; The transport of citrate and other tricarboxylic acids in two species of Pseudomonas. Biochem J 1 July 1971; 123 (4): 571–577. doi: https://doi.org/10.1042/bj1230571
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