1. Heparin, heparan sulphate, chondroitin sulphate and dermatan sulphate were covalently attached to beads of agarose activated by cyanogen bromide. The bond is probably mediated by the amino group of a serine or peptide residue at the reducing end of the polysaccharide chain. 2. The uptake of glycosaminoglycan during the coupling procedure is about 0.9mg/ml of wet gel. However, direct analysis of washed and freeze-dried gels reveals that only about one-third of this amount is firmly attached to the gel. 3. The use of the gels for polysaccharidase analyses is exemplified by a hyaluronidase assay. Further applications, e.g. interaction studies and preparative purposes, are discussed.
Research Article|October 01 1971
Coupling of glycosaminoglycans to agarose beads (Sepharose 4B)
Biochem J (1971) 124 (4): 677-683.
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P.-H. Iverius; Coupling of glycosaminoglycans to agarose beads (Sepharose 4B). Biochem J 1 October 1971; 124 (4): 677–683. doi: https://doi.org/10.1042/bj1240677
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