Yolk-sacs were removed from 17.5-day pregnant rats injected 2–5h previously with 125I-labelled bovine serum albumin. The specific activities of acid phosphatase and acid proteinase, and the specific radioactivities (trichloroacetic acid-insoluble and trichloroacetic acid-soluble) were measured in subcellular fractions prepared by homogenization and differential centrifugation. The conversion of acid-insoluble into acid-soluble radioactivity within cultured tissue was followed and the nature of the liberated products was investigated by gel chromatography. The results are consistent with the protein entering lysosomes and being digested there. The radiolabel was released chiefly as free iodotyrosine.
Research Article|November 01 1971
Digestion of an exogenous protein by rat yolksac cultured in vitro
K. E. Williams;
J. B. Lloyd;
Biochem J (1971) 125 (1): 303-308.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
K. E. Williams, J. B. Lloyd, M. Davies, F. Beck; Digestion of an exogenous protein by rat yolksac cultured in vitro. Biochem J 1 November 1971; 125 (1): 303–308. doi: https://doi.org/10.1042/bj1250303
Download citation file: