The mechanism of long-chain fatty acid activation catalysed by highly purified microsomal palmitoyl-CoA synthetase was investigated. The kinetics of the overall reaction were found to conform to the Bi Uni Uni Bi Ping Pong mechanism. 18O was transferred from [18O]palmitate to AMP and palmitoyl-CoA exclusively. The enzyme intermediate formed appeared to consist of enzyme-bound palmitate; this formation occurred only in the presence of ATP. However, the involvement of palmitoyl-AMP in the reaction catalysed by the purified enzyme has proved difficult to establish.
Research Article| February 01 1973
Palmitoyl-coenzyme A synthetase. Mechanism of reaction
Biochem J (1973) 131 (2): 199–209.
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J. Bar–Tana, G. Rose, R. Brandes, B. Shapiro; Palmitoyl-coenzyme A synthetase. Mechanism of reaction. Biochem J 1 February 1973; 131 (2): 199–209. doi: https://doi.org/10.1042/bj1310199
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