A method is described for the preparation of mitochondria from the slime mould Physarum polycephalum; the mitochondria were not coupled. P. polycephalum mitochondria oxidized added NADH via a rotenone-insensitive pathway, but the oxidation of malate plus glutamate was rotenone sensitive; both of these substrates reduced much less cytochrome b than did succinate, in both aerobic and anaerobic steady states. Spectroscopy at 77°K separated three absorption maxima in the α-band region, at 560nm, 553nm and one at 547nm due to cytochrome c. The absorption at 553nm was increased in the aerobic steady state by the addition of 2-heptyl-4-hydroxyquinoline N-oxide, suggesting that it was due to a b-type cytochrome. All three absorption maxima appeared in the aerobic steady state after the addition of a range of substrates. The respiratory activity with different substrates and the response to inhibitors of respiration were similar to those previously described for fungus mitochondria (Weiss et al., 1970; Erickson & Ashworth, 1969). When grown under conditions of haem limitation the mitochondria contained a lower concentration of cytochromes than normal.
Research Article|July 15 1973
The electron-transport system of mitochondria from the slime mould Physarum polycephalum
Emer M. Colleran;
Biochem J (1973) 134 (3): 745-751.
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Roger Barnes, Emer M. Colleran, O. T. G. Jones; The electron-transport system of mitochondria from the slime mould Physarum polycephalum. Biochem J 15 July 1973; 134 (3): 745–751. doi: https://doi.org/10.1042/bj1340745
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