1. The choline dehydrogenase (EC 126.96.36.199) WAS SOLUBILIZED FROM ACETONE-DRIED POWDERS OF RAT LIVER MITOCHONDRIA BY TREATMENT WITH Naja naja venom. 2. The kinetics of the reaction of enzyme with phenazine methosulphate and ubiquinone-2 as electron acceptors were investigated. 3. With both electron acceptors the reaction mechanism appears to involve a free, modified-enzyme intermediate. 4. With some electron acceptors the maximum velocity of the reaction is independent of the nature of the acceptor. With phenazine methosulphate and ubiquinone-2 as acceptors the Km value for choline is also independent of the nature of the acceptor molecule. 5. The mechanism of the Triton X-100-solubilized enzyme is apparently the smae as that for the snake venom solubilized enzyme.
Research Article|December 01 1975
The reaction of choline dehydrogenase with some electron acceptors
M C Barrett;
Biochem J (1975) 151 (3): 677-683.
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M C Barrett, A P Dawson; The reaction of choline dehydrogenase with some electron acceptors. Biochem J 1 December 1975; 151 (3): 677–683. doi: https://doi.org/10.1042/bj1510677
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