1. Pulmonary surfactants from ox, rabbit, rat and sheep were isolated and analysed. 2. All preparations had a high anenoic phosphatidylcholine content and would produce stable surface tensions of 0.01 Nm−1 or less. 3. Protein content was 8-18% of the dry weights. A number of proteins were observed; their overall composition were high in hydrophobic amino acid residues. 4. Lipid content varied from 79% (ox) to 90% (rabbit) with phosphatidylcholine representing from 58% (sheep) to 83% (rabbit) of the total lipid. The surfactant preparations were rather similar in lipid composition except that sheep surfactant contained about 10% lysophosphatidylcholine. 5. Hexadecanoic acid was the principal fatty acid. It was particularly high in phosphatidylcholine. 6. Phosphatidylglycerol was a minor constituent of all surfactants but phosphatidyldimethylethanolamine was not detected.
Research Article|December 01 1975
Characterization of pulmonary surfactant from ox, rabbit, rat and sheep
J L Harwood;
Biochem J (1975) 151 (3): 707-714.
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J L Harwood, R Desai, P Hext, T Tetley, R Richards; Characterization of pulmonary surfactant from ox, rabbit, rat and sheep. Biochem J 1 December 1975; 151 (3): 707–714. doi: https://doi.org/10.1042/bj1510707
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