In rat liver mitochondria suspended in KC1 medium, oligomycin interfered with the effect of uncouplers on energy conservation. It antagonized the effect of uncouplers that are weak acids (2,4-dinitrophenol etc.), but enhanced that of the lipid-penetrating cation NN-dimethyl-N'N'-dibenzylammonium. Oligomycin caused none of the above effects when Br− or NO−/3 was substituted for C1− as the major anionic species in the assay medium. The concentration of oligomycin that exerted the above-mentioned effects was lower than that necessary for the inhibition of energy transfer, but was in the range that induced C1− permeation through the cristae membrane. The possible connexion between the effect of oligomycin on C1− permeation and its interference with the action of uncouplers is discussed.
Research Article|October 01 1975
Chloride-dependent restoration of coupling by oligomycin in rat liver mitochondria
Biochem J (1975) 152 (1): 115-119.
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N Ariel, Y Avi-Dor; Chloride-dependent restoration of coupling by oligomycin in rat liver mitochondria. Biochem J 1 October 1975; 152 (1): 115–119. doi: https://doi.org/10.1042/bj1520115
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