The purification of guinea-pig intestinal brush borders by a rapid sucrose-gradient-centrifugation step is reported. A 29-fold increase in the maltase/DNA quotient indicates considerable purification of the brush borders from nuclei. The biological activity of the brush borders was well preserved, as demonstrated by a high recovery of human gastric-juice-mediated uptake of 57Co-labelled vitamin B-12; homogeneity and purity were confirmed by scanning electron microscopy. Both the morphological appearance and biological activity were unchanged after prolonged storage in glycerol.
Research Article|October 01 1975
Purification and characterization of guinea-pig intestinal brush borders
G V Lippe;
Biochem J (1975) 152 (1): 157-159.
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K Andersen, G V Lippe, L Morkrid, H Schjonsby; Purification and characterization of guinea-pig intestinal brush borders. Biochem J 1 October 1975; 152 (1): 157–159. doi: https://doi.org/10.1042/bj1520157
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