Novikoff hepatoma ascites cells, grown intraperitoneally in rats, are shown to possess a high-affinity, low-capacity dexamethasone-binding protein. The receptor protein has an intracellular localization and concentration, association constant (Ka), glucocorticoid specificity and nuclear uptake in the presence of dexamethasone comparable with those of the G-protein of rat liver. During therapy of the tumour-bearing animal with cortisol, marked cyclic variations were observed in the concentration and nuclear uptake of the putative G-protein in the tumor cells; more transient variations were also observed in the Ka value of the receptor protein.
The dexamethasone receptor in the Novikoff Hepatoma. Characterization and changes in concentration and nuclear uptake during glucocorticoid therapy
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S L Liu, D J McNamara, T E Webb; The dexamethasone receptor in the Novikoff Hepatoma. Characterization and changes in concentration and nuclear uptake during glucocorticoid therapy. Biochem J 1 December 1975; 152 (3): 689–695. doi: https://doi.org/10.1042/bj1520689
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