Raman polarization measurements of the amide I band are reported in ionized poly-L-lysine dissolved in aqueous methanol. The observed changes with methanol concentration, attributed to changes in coil conformation and to the helix-coil transition, represent a novel method of measuring polymer conformation. Polarization measurements as a function of temperature yield values of the energy differences between rotational isomeric states in the coil. deltaH, of 8.8 +/- 0.7, 10.4 +/- 1.1 and 10.8 +/- 1.5 kJ/mol at methanol concentrations (v/v) of 85, 80 and 70% respectively. The stabilization energy of the helix is estimated at 9.3 kJ/mol.
Research Article|June 01 1976
Study of poly-L-lysine conformations in aqueous methanol solution by using polarized Raman techniques
Biochem J (1976) 155 (3): 543-548.
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I W Shepherd; Study of poly-L-lysine conformations in aqueous methanol solution by using polarized Raman techniques. Biochem J 1 June 1976; 155 (3): 543–548. doi: https://doi.org/10.1042/bj1550543
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