Bovine ligamentum nuchae elastin that had been partially digested by porcine pancreatic elastase and then thoroughly washed with 0.2 M-NaC1/0.05 M-sodium borate solution continued to release peptide fragments in solution. Complete removal of elastase from the elastin surface required extraction with dilute alkali, a procedure that does not irreversibly inactivate the enzyme or hydrolyse elastin.
Research Article|June 01 1976
Removal of adsorbed elastase from partially digested elastin
H P Fromageot;
Biochem J (1976) 155 (3): 705-707.
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H P Fromageot, G M Slusarczuk; Removal of adsorbed elastase from partially digested elastin. Biochem J 1 June 1976; 155 (3): 705–707. doi: https://doi.org/10.1042/bj1550705
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