The enzyme cholesterol ester hydrolase (EC 18.104.22.168) was detected in the larvae of the khapra beetle, Trogoderma granarium (Everts). The pH and temperature optima for the enzyme were 6.6 degrees and 37 degrees C respectively. The mol.wt. of the enzyme was 76000-80000. The enzyme was equally effective in hydrolysing cholesteryl acetate, stearate and oleate. Cholesterol derivatives, namely the chloride and the methyl ether, inhibited the enzyme activity almost completely. It was also inhibited completely by p-hydroxymercuribenzoate. This inhibition was reversed by the addition of dithiothreitol, reduced glutathione or cysteine. The enzyme activity was associated predominantly with the 104000 g fraction.
Research Article|July 01 1976
Studies on the cholesterol ester hydrolase of Trogoderma (Coleoptera)
Biochem J (1976) 157 (1): 111-116.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
H C Agarwal, A M G Nair; Studies on the cholesterol ester hydrolase of Trogoderma (Coleoptera). Biochem J 1 July 1976; 157 (1): 111–116. doi: https://doi.org/10.1042/bj1570111
Download citation file: