The reaction of [nitrotyrosyl]cytochrome c with ligands was studied by stopped-flow techniques. At pH 7.0 the reaction with imidazole shows two distinct phases, one fast phase being concentration-dependent and a slow phase being concentration-independent. The results are consistent with the existence of two forms of [nitrotyrosyl]cytochrome c in solutions [Schejter et al. (1970) Biochemistry 9, 5118-5122]; form I, the smaller fraction, seems to be responsible for the slow first-order process.
Research Article|July 01 1976
Kinetics of reaction of [nitrotyrosyl]cytochrome c with ligands
Biochem J (1976) 157 (1): 217-220.
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A José do Nascimento, K Hishida do Nascimento; Kinetics of reaction of [nitrotyrosyl]cytochrome c with ligands. Biochem J 1 July 1976; 157 (1): 217–220. doi: https://doi.org/10.1042/bj1570217
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