Fluorescence of 8-anilinonaphthalene-1-sulphonate is enhanced by both lactoferrin and transferrin. The enhancement is relatively low between pH 4 and 8, and high at below pH 4. At physiological pH the fluorescence enhancement is higher with the iron-deprived than with the iron-saturated proteins. Binding of iron by lactoferrin is associated with lowering affinity for the dye.
Research Article|July 01 1976
Iron-dependent binding of 8-anilinonaphthalene-1-sulphonate by both lactoferrin and transferrin
Biochem J (1976) 157 (1): 233-235.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
N Bilić, M Casey, B Blanc; Iron-dependent binding of 8-anilinonaphthalene-1-sulphonate by both lactoferrin and transferrin. Biochem J 1 July 1976; 157 (1): 233–235. doi: https://doi.org/10.1042/bj1570233
Download citation file: