Z discs were isolated from Lethocerus flight muscle by removing the contractile proteins from myofibrils with a solution of high ionic strength. The protein composition of the Z discs was analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis; the major proteins were alpha-actinin, actin and tropomyosin. Z lines were selectively removed from intact myofibrils by digestion with crude lipase and chymotrypsin, but not by purified lipase.
Research Article|February 01 1977
The proteins in the Z line of insect flight muscle
Biochem J (1977) 161 (2): 399-403.
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B Bullard, G M Sainsbury; The proteins in the Z line of insect flight muscle. Biochem J 1 February 1977; 161 (2): 399–403. doi: https://doi.org/10.1042/bj1610399
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