The sigma-factor of Escherichia coli RNA polymerase was shown to dissociate from the core enzyme as a function of absolute concentration. The association constant is in the range 10(6)-10(8) litre/mol. This implies that the amount of holoenzyme, core enzyme and sigma-factor in RNA polymerase assays may vary according to the absolute concentration of the enzyme.
Research Article|April 01 1977
The dissociation of σ-factor from ribonucleic acid polymerase
Biochem J (1977) 163 (1): 177-179.
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A M Campbell, P A Lowe; The dissociation of σ-factor from ribonucleic acid polymerase. Biochem J 1 April 1977; 163 (1): 177–179. doi: https://doi.org/10.1042/bj1630177
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