1. During growth of Pseudomonas phenazinium on l-threonine medium, phenazine pigment formation commenced early and 1,6-dihydroxyphenazine 5,10-dioxide (iodinin) was the major component. Growth on l-[U-14C]threonine showed that when growth was complete about 25% of the label had been incorporated into phenazines and 30% into cell substance. 2. The addition of d-[2,3,4,5(n)-14C]shikimate to cultures at different phases of growth showed that the greatest efficiency of incorporation (about 70%) occurred in the mid- to late-exponential phase. Phenazines accounting for most of the 14C supplied were iodinin and 9-hydroxyphenazine-1-carboxylate plus 2,9-dihydroxyphenazine-1-carboxylate. Radioactivity incorporated into cell substance was about one-third of the amount found in phenazines. 3. Kinetic studies showed that radioactivity from a pulse of [14C]-shikimate was incorporated into phenazines immediately, without a discernible lag, and into all detectable phenazines simultaneously rather than sequentially. 4. Radioactive phenazines isolated from culture media were fed to growing cultures and their metabolism was studied. The results supported a scheme for the biosynthesis of iodinin and 1,8-dihydroxyphenazine 10-monoxide by a branched pathway. 5. It is proposed that phenazine-1,6-dicarboxylate is the common precursor of all naturally occurring phenazines.
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Research Article|
April 15 1977
Incorporation of [14C]shikimate into phenazines and their further metabolism by Pseudomonas phenazinium
Graham S. Byng;
Graham S. Byng
1Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, U.K.
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John M. Turner
John M. Turner
1Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, U.K.
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Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1977 London: The Biochemical Society
1977
Biochem J (1977) 164 (1): 139–145.
Citation
Graham S. Byng, John M. Turner; Incorporation of [14C]shikimate into phenazines and their further metabolism by Pseudomonas phenazinium. Biochem J 15 April 1977; 164 (1): 139–145. doi: https://doi.org/10.1042/bj1640139
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