At constant 1 mM-ATP, the Mg2+-saturation curves for adenylate cyclase (EC 18.104.22.168) particulate preparations obtained from corpus striatum and cortex tissues of rat brain show that addition of 0.1 mM-noradrenaline increases the apparent Vmax. for Mg2+ by 300% in corpus striatum particles, and by 280% in cortex particles. At 10 mM-MgCl2, the addition of 0.1 mM-noradrenaline increased by 800% the adenylate cyclase activity of corpus striatum particles. At all Mg2+ concentrations, the addition of 0.3 mM-CaCl2 suppressed the noradrenaline-induced stimulation of adenylate cyclase of corpus striatum particles, and even resulted in a strong inhibition of the activating effect of Mg2+ itself on adenylate cyclase of corpus striatum particles, and even resulted in a strong inhibition of the activating effect of Mg2+ itself on adenylate cyclase activity of cortex particles. The addition of noradrenaline during a 3 h preincubation of particle preparations of brain cortex at 38 degrees C decreased by more than 4-fold the half-life of the decay of adenylate cyclase activity. The addition of MgATP protected against noradrenaline-induced inactivation.
Research Article|September 15 1977
Effects of noradrenaline on the activation and the stability of brain adenylate cyclase
Biochem J (1977) 166 (3): 473-483.
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S Brydon-Golz, H Ohanian, A Bennun; Effects of noradrenaline on the activation and the stability of brain adenylate cyclase. Biochem J 15 September 1977; 166 (3): 473–483. doi: https://doi.org/10.1042/bj1660473
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