Orientation of rat renal and intestinal brush-border membrane vesicles was studied with two independent methods: electron-microscopic freeze-fracture technique and immunological methods. With the freeze-fracture technique a distinct asymmetric distribution of particles on the two membrane fracture faces was demonstrated; this was used as a criterion for orientation of the isolated membrane vesicles. For the immunological approach the accessibility or inaccessibility of aminopeptidase M localized on the outer surface of the cell membrane to antibodies was used. With both methods we showed that the brush-border membrane vesicles isolated from rat kidney cortex and from rat small intestine for transport studies are predominantly orientated right-side out.
Research Article|April 15 1978
Studies on the orientation of brush-border membrane vesicles
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Biochem J (1978) 172 (1): 57-62.
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W Haase, A Schäfer, H Murer, R Kinne; Studies on the orientation of brush-border membrane vesicles. Biochem J 15 April 1978; 172 (1): 57–62. doi: https://doi.org/10.1042/bj1720057
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