A rapid (less than 2h) method is described for the preparation of synaptosomes from rat brain by using a discontinuous Ficoll/sucrose gradient by a flotation technique. These synaptosomes are metabolically active and minimally (less than 5%) contaminated with ‘free’ mitochondria as judged by marker-enzyme assays and electron microscopy.
Research Article| November 15 1978
A rapid method for the preparation of relatively pure metabolically competent synaptosomes from rat brain
Biochem J (1978) 176 (2): 365–370.
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R F G Booth, J B Clark; A rapid method for the preparation of relatively pure metabolically competent synaptosomes from rat brain. Biochem J 15 November 1978; 176 (2): 365–370. doi: https://doi.org/10.1042/bj1760365
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