Mitochondria were isolated from mature leaves as well as etiolated seedlings of Brassica campestris (mustard), a eukaryote previously shown to possess the iron-containing isoenzyme of superoxide dismutase. On the basis of KCN- and H2O2-sensitivity, and on polyacrylamide-gel analysis, only the cuprozinc and mangano superoxide dismutases were found in mitochondria. The iron-containing enzyme was absent. The mangano enzyme was found in the mitochondrial matrix, whereas the cuprozinc enzyme appeared to be localized in the intermembrane space.
Research Article|April 01 1981
Absence of the iron-containing superoxide dismutase in mitochondria from mustard (Brassica campestris)
Biochem J (1981) 195 (1): 229-233.
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M L Salin, S M Bridges; Absence of the iron-containing superoxide dismutase in mitochondria from mustard (Brassica campestris). Biochem J 1 April 1981; 195 (1): 229–233. doi: https://doi.org/10.1042/bj1950229
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