A population of sarcoplasmic-reticulum vesicles, all of which were sealed with their cytoplasmic side outwards, was obtained by density-gradient centrifugation after loading the vesicles with calcium oxalate. The calcium oxalate could subsequently be removed from the vesicles by the reverse action of the calcium-transport system. Measurements of the catalysed exchange of the phosphatidylcholine in the sarcoplasmic-reticulum cytoplasmic monolayer with an exogenous phosphatidylcholine pool suggested that phosphatidylcholine is symmetrically distributed across the sarcoplasmic-reticulum membrane. A similar result was obtained for phosphatidylethanolamine when sarcoplasmic-reticulum lipids were labelled with trinitrobenzenesulphonic acid. Further catalysed lipid-exchange reactions showed that the transverse movement of phosphatidylcholine across the membrane was exceedingly slow (t 1/2 greater than 15 days).
Transbilayer distribution of lipids in a population of sarcoplasmic-reticulum vesicles sealed with their cytoplasmic side outwards
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K A McGill, J P Bennett, G A Smith, R W Plumb, G B Warren; Transbilayer distribution of lipids in a population of sarcoplasmic-reticulum vesicles sealed with their cytoplasmic side outwards. Biochem J 1 April 1981; 195 (1): 287–295. doi: https://doi.org/10.1042/bj1950287
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