1. Quantitative O-deacylation of phospholipids has been achieved by incubation with a reagent containing monomethylamine, methanol and water. The reaction is primarily an O leads to N-transacylation with N-methyl fatty acid amides being formed. 2. The reagent can be removed easily by volatilization and under defined conditions no secondary decomposition of the phosphorus-containing deacylation products occurs. 3. The water-soluble phosphorus compounds derived by deacylation of mammalian tissue O-diacylated phospholipids have been completely separated by a single-dimensional paper ionophoresis with a volatile pH9 buffer. 4. The O-deacylated alkyl and alkenyl phospholipids have been examined by t.l.c. before and after catalytic hydrolysis with Hg2+. 5. A complete analysis of rat brain phospholipids by the above methods agrees closely with that obtained by other procedures.
Research Article|April 01 1981
Alkaline O→N-transacylation. A new method for the quantitative deacylation of phospholipids
Biochem J (1981) 195 (1): 301-306.
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N G Clarke, R M C Dawson; Alkaline O→N-transacylation. A new method for the quantitative deacylation of phospholipids. Biochem J 1 April 1981; 195 (1): 301–306. doi: https://doi.org/10.1042/bj1950301
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