The very-low-density-lipoprotein secretion rate of isolated hepatocytes obtained from rats fed a high-fat diet was half that of cells from control animals. In fat-fed rats, the initial cellular uptake of [l-14C]oleate in vitro was decreased by 25%, its esterification to triacylglycerols and phospholipids by 50% and its incorporation into very-low-density-lipoprotein triacylglycerols by 70%. Exogenous oleate was not the main precursor of very-low-density lipoproteins in these animals. Lipogenesis, a minor source of very-low-density lipoproteins with the control diet in our experimental conditions, was inhibited by 84% after fat-feeding. A short-term inhibition of lipogenesis in vitro did not result in a decrease in very-low-density-lipoprotein secretion rate. The results suggest that fat-feeding decreased availability of exogenous as well as endogenous fatty acids for synthesis of very-low-density lipoproteins.
Research Article|August 15 1981
Very-low-density-lipoprotein secretion by isolated hepatocytes of fat-fed rats
A D Kalopissis; ; ; ;
Biochem J (1981) 198 (2): 373-377.
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A D Kalopissis, S Griglio, M I Malewiak, R Rozen, X L Liepvre; Very-low-density-lipoprotein secretion by isolated hepatocytes of fat-fed rats. Biochem J 15 August 1981; 198 (2): 373–377. doi: https://doi.org/10.1042/bj1980373
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