When freshly prepared glucocorticoid-receptor complex from rat liver cytosol was incubated at 23 degrees C in the presence of sodium molybdate, its subsequent binding to isolated nuclei, DNA-cellulose and ATP-Sepharose was blocked. In addition, binding to these acceptors by cytosol receptor complex fractionated with (NH4)2SO4 was also blocked by incubation of the complexes with 50 mM-sodium molybdate. However, molybdate had no effect on the binding of activated receptor complexes to ATP-Sepharose. Molybdate was also effective in extracting the nuclear- and DNA-cellulose-bound glucocorticoid-receptor complexes in a dose-dependent manner. Molybdate appears to exert its effects directly on the receptor by interacting with both non-activated and activated receptor forms.
Inactivation of rat liver glucocorticoid receptor by molybdate. Effects on both non-activated and activated receptor complexes
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N Murakami, V K Moudgil; Inactivation of rat liver glucocorticoid receptor by molybdate. Effects on both non-activated and activated receptor complexes. Biochem J 15 September 1981; 198 (3): 447–455. doi: https://doi.org/10.1042/bj1980447
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