Analysis of the soluble protein fractions from the rat parotid, submandibular and sublingual glands by polyacrylamide-gel electrophoresis reveals similarities in overall patterns of protein synthesis at birth. Tissue-specific changes in protein and glycoprotein synthesis occur shortly after birth and again at the time of weaning, 21-28 days later. Incorporation of [3H]thymidine into DNA was at its highest after birth and gradually decreased in both the parotid and submandibular gland, whereas [3H]thymidine incorporation in the sublingual gland was low throughout the time of neonatal development. [14C]Leucine incorporation into total protein increased in all glands with age after birth, showing an accelerated rate 21-28 days later. Trichloroacetic acid/phosphotungstic acid-precipitable [3H]fucose in glycoproteins declined over the time of neonatal development in the parotid and submandibular gland, but its incorporation remained higher in the sublingual gland. alpha-Amylase (EC 188.8.131.52) in the salivary glands increased at the time of weaning, as judged by detectability in sodium dodecyl sulphate/polyacrylamide gels and by immune precipitation. Two membrane-bound enzymes, UDP-galactose:2-acetamido-2-deoxy-D-glucosamine 4 β-galactosyltransferase (EC 184.108.40.206) and UDP-galactose:2-acetamido-2-deoxy-D-galactosaminyl-protein 3 β-galactosyltransferase (no EC number), undergo tissue-specific change rather than changes induced by physiological stimulation of the salivary glands.
Comparative developmental analysis of the parotid, submandibular and sublingual glands in the neonatal rat
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M G Humphreys-Beher, D L Hollis, D M Carlson; Comparative developmental analysis of the parotid, submandibular and sublingual glands in the neonatal rat. Biochem J 15 June 1982; 204 (3): 673–679. doi: https://doi.org/10.1042/bj2040673
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