After adaptation of rats to a 90%-casein diet, hepatic uptake of alanine is strikingly increased in vivo, with concomitant appearance of a concentration of favourable for uptake. With a high-protein diet, uptake of 2-aminoisobutyrate by isolated hepatocytes in the presence of various concentrations of substrates suggested induction of the A system (high-affinity system), whose emergence has been reported during starvation or after glucagon treatment. The other system (ASC, L) were characterized: induction processes only affected the A system. Dibutyryl cyclic AMP addition resulted in an increase in 2-aminoisobutyrate transport at low substrate concentration, the response being greater after adaptation to a high-protein diet. Evidence is presented suggesting that the increased uptake of amino acids by the liver of rats fed on high-protein diets is obtained by developing favourable gradients and enhancing transport capacities. These adaptations allow sufficient amounts of amino acids to enter the liver, where accelerated metabolism plays a decisive role.
Research Article|July 15 1982
Stimulation of amino acid transport into liver cells from rats adapted to a high-protein diet
Biochem J (1982) 206 (1): 13-18.
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P Fafournoux, C Rémésy, C Demigné; Stimulation of amino acid transport into liver cells from rats adapted to a high-protein diet. Biochem J 15 July 1982; 206 (1): 13–18. doi: https://doi.org/10.1042/bj2060013
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